The 5th Indonesian Society of Limnology (MLI) Congress and International Conference 2021

The 5th Indonesian Society of Limnology (MLI) Congress and International Conference 2021 is a biannual conference organized by the Indonesian Society of Limnology, with the theme is “Building synergies towards sustainable use of inland waters.” This year due to the COVID-19 pandemic, we hold the event virtually from 2nd to 3rd December. The objectives of this event are to (1) Connect, discuss, share and create a mutual network among communities from different backgrounds who are interested in inland waters ecosystem;(2) Disseminate science & technology and lessen the gap between scientific and common communities through fruitful discussion settings;(3) Underpin sustainable use and management of inland aquatic ecosystems.There were four keynotes speakers, four invited speakers, and 66 general presenters in the conference sessions. In total, 116 participants were registered and joined the conference. The first keynote speaker was Prof. Dr. Gadis Sri Haryani from the Research Center for Limnology and Water Resources, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Indonesia, who presented research about Migratory freshwater fish in Indonesia: Threats and conservation efforts. The second presenter was Dr. Robert Walsh from the Australian Water Life, Australia, who presented research about Discover the world of Micro-invertebrates. The third keynote speaker was Dr. Khamla Inkhavilay from the National University of Laos, Lao PDR, who presented Persistent Organic Pollutants in Wetland of Mekong Basin. The fourth speaker was Dr. Kwanraree Joy Sirikanchana from the Chulabhorn Research Institute, Thailand, who presented Microbial Source Tracking and Quantitative Microbial Risk Assessment for Sustainable Water Pollution Management.In addition, the four invited speakers and 66 general presenters split into four rooms for parallel discussions which covered the latest research on inland water ecosystems, including;(1) Biotic resources, biodiversity, and conservation;(2) System Dynamic of inland waters;(3) Applied technology for the management and pollution control;(4) Modelling, system information, decision support tool, disaster risk reduction;(5) Management, policies, regulation, education, social, economy, and culture.We highly appreciate the generous support from Research Centre for Limnology-BRIN (Indonesia), Australian Water Life (Australia), Chulabhorn Research Institute (Thailand), National University of Laos (Lao PDR), Southeast Asian Limnological Network (SEALnet), Advisory Board, Steering and Organizing committee and all presenters and participants.List of Committees, Advisory Board, Steering Committee, Scientific Committee, Organizing Committee, Documentation, all photos are available in this pdf.

Six months in the Lhù'ààn Mân' (Kluane Lake) watershed: Autobiographical reflection on the benefits of an extended data collection campaign

Lhù'ààn Mân' is located in the southwest corner of Yukon Territory, tucked into the foot of Kluane Ranges of the St. Elias Mountains. The lake is situated on the traditional territory of the Kluane First Nation, Champagne and Aishihik First Nations, and the White River First Nation. The lake and its watershed are culturally significant and provide sources of fresh water, fish, land animals for hunting and trapping, berries, and lumber. Miller is a a PhD candidate in the Department of Geography at the University of Calgary working on an exploratory hydrology research project in the Lhù'ààn Mân' watershed. In May of 2016, Kaskawulsh Glacier retreat redirected the meltwater away from it. The event caused the volume of water to decrease significantly, leaving the areas of the river valley that were previously under water dry. Kaskawulsh Glacier runoff was the largest source of glacial water to the lake until then. By August, its level dropped nearly 2 m and has not refilled. This drastic change over a short time period drew a lot of attention and has raised questions in the academic and local communities about glacially-connected water resources in a changing climate.

Consumers' purchase intention of wild freshwater fish during the COVID-19 pandemic.

The association between the COVID-19 pandemic and the wildlife trade in the seafood market in Wuhan has raised public concern regarding wildlife consumption and public health safety. Considering several coronavirus transmission incidents related to aquatic products and the location of wild freshwater fish in aquatic consumption in China, the effects of COVID-19 on the purchase intention of wild freshwater fish was investigated. Based on 1163 online questionnaires from eight provinces (including two province-level municipalities) in the Yangtze River Basin, ordered logistic regression was carried out to analyze the influencing factors of purchase intention of wild freshwater fish during the COVID-19 pandemic. The empirical results indicated that the COVID-19 pandemic had changed consumers' perceived risk and purchase frequency of wild freshwater fish. External stimulus caused by the COVID-19 pandemic had little influence on perceived risk and purchase intention. Consumer preference had a significant impact on perceived risk and purchase intention. Therefore, efforts should be put to strengthen the popularization of aquatic product knowledge, guide the public to develop scientific and civic eating habits, and improve the traceability system of aquatic products. [EconLit Citations: D12-Consumer Economics: Empirical Analysis, Q22-Fishery; Aquaculture].

A global perspective on the influence of the COVID-19 pandemic on freshwater fish biodiversity. (Special Issue: Conservation and ecological impacts of the COVID-19 pandemic.)

The COVID-19 global pandemic and resulting effects on the economy and society (e.g., sheltering-in-place, alterations in transportation, changes in consumer behaviour, loss of employment) have yielded some benefits and risks to biodiversity. Here, we considered the ways the COVID-19 pandemic has influenced (or may influence) freshwater fish biodiversity (e.g., richness, abundance). In many cases, we could only consider potential impacts using documented examples (often from the media) of likely changes, because anecdotal observations are still emerging and data-driven studies are yet to be completed or even undertaken. We evaluated the potential for the pandemic to either mitigate or amplify widely acknowledged, pre-existing threats to freshwater fish biodiversity (i.e., invasive species, pollution, fragmentation, flow alteration, habitat loss and alteration, climate change, exploitation). Indeed, we identified examples spanning the extremes of positive and negative outcomes for almost all known threats. We also considered the pandemic's impact on freshwater fisheries demand, assessment, research, compliance monitoring, and management interventions (e.g., restoration), with disruptions being experienced in all domains. Importantly, we provide a forward-looking synthesis that considers the potential mechanisms and pathways by which the consequences of the pandemic may positively and negatively impact freshwater fishes over the longer term. We conclude with a candid assessment of the current management and policy responses and the extent to which they ensure freshwater fish populations and biodiversity are conserved for human and aquatic ecosystem benefits in perpetuity.

Comparison of physicochemical properties of different tissues from China climbing perch Anabas testudineus and crucian carp Carassius auratus.

This study aimed to investigate nutrition in climbing perch Anabas testudineus which is an important nutritious economic freshwater fish in Asia and compare with Carassius auratus (crucian carp). Three kinds of tissues, including muscle, livers, and eggs, were isolated, respectively. Physicochemical properties including moisture, ash, protein, amino acids, fat, vitamins, and calcium contents in those tissues were determined. The results showed climbing perch muscle and liver contained less moisture, but more protein, amino acids, and vitamins than crucian carp muscle and liver. While moisture, ash, protein, and total amino acids contents of climbing perch egg were lower than those of crucian carp egg. Climbing perch egg had more fat, vitamins, and calcium than crucian carp egg. The amino acid profile was also performed, and 16 amino acids were identified and quantified in muscle, liver, and egg. Among tissues, the highest and lowest concentration of total amino acid content was found in crucian carp eggs and livers, respectively. Glutamic acid (Glu) and histidine (His) were the most and least amino acids in climbing perch and crucian carp tissues, respectively. Sixteen amino acids in climbing perch egg were less than those in crucian carp egg while it is an opposite case in muscle and liver, which amino acids of climbing perch tissues were more than those of crucian carp muscle and liver. Vitamin A of climbing perch was more than crucian carp in all three tissues, but vitamin E content in climbing perch liver was lower than that of crucian carp liver. Calcium content of muscle had no difference between two species. The abovementioned comparison of physicochemical properties of different tissues from China climbing perch and crucian carp will provide a necessary supplementary of freshwater muscle nutrition research, also was helpful for application of climbing perch.

Can spike fragments of SARS-CoV-2 induce genomic instability and DNA damage in the guppy, Poecilia reticulate? An unexpected effect of the COVID-19 pandemic.

The identification of SARS-CoV-2 particles in wastewater and freshwater ecosystems has raised concerns about its possible impacts on non-target aquatic organisms. In this particular, our knowledge of such impacts is still limited, and little attention has been given to this issue. Hence, in our study, we aimed to evaluate the possible induction of mutagenic (via micronucleus test) and genotoxic (via single cell gel electrophoresis assay, comet assay) effects in Poecilia reticulata adults exposed to fragments of the Spike protein of the new coronavirus at the level of 40 µg/L, denominated PSPD-2002. As a result, after 10 days of exposure, we have found that animals exposed to the peptides demonstrated an increase in the frequency of erythrocytic nuclear alteration (ENA) and all parameters assessed in the comet assay (length tail, %DNA in tail and Olive tail moment), suggesting that PSPD-2002 peptides were able to cause genomic instability and erythrocyte DNA damage. Besides, these effects were significantly correlated with the increase in lipid peroxidation processes [inferred by the high levels of malondialdehyde (MDA)] reported in the brain and liver of P. reticulata and with the reduction of the superoxide dismutase (SOD) and catalase (CAT) activity. Thus, our study constitutes a new insight and promising investigation into the toxicity associated with the dispersal of SARS-CoV-2 peptide fragments in freshwater environments.

Differential Molecular Responses of Zebrafish Larvae to Fluoxetine and Norfluoxetine

The occurrence of psychopharmaceuticals in aquatic ecosystems is a growing problem. Fluoxetine (FL) and its metabolite norfluoxetine (NF) are selective serotonin reuptake inhibitors. Although they may be potentially harmful to non-target species, available knowledge on the effects of NF is sparse, relative to FL. This study aimed at contributing to the body of knowledge about the modes-of-action (MoA) of these compounds and their underlying mechanisms eliciting hazardous effects during the early development of the teleost model zebrafish (Danio rerio). One hour post-fertilisation (hpf), embryos were exposed up to 80 hpf to these compounds at levels found in surface waters and higher (FL, 0.0015 and 0.05 µM;NF, 0.00006 and 0.0014 µM). Developmental anomalies were observed at 8, 32 and 80 hpf. Larvae were collected at 80 hpf to assess the expression of 34 genes related to FL and NF MoA and metabolism, using qPCR (quantitative reverse transcription PCR). Results showed that both compounds elicited an increased frequency of embryos exhibiting abnormal pigmentation, relative to controls. Gene expression alterations were more pronounced in FL- than in NF-exposed larvae. Cluster Analysis revealed two groups of genes discriminating between the drugs. for their marked opposing responses. Globally, downregulation of gene expression was typical of FL, whilst upregulation or no alteration was found for NF. These clusters identified were linked to the adrenergic pathway and to the retinoid and peroxisome proliferator-activated nuclear receptors. Overall, our data contradict the prevailing notion that NF is more toxic than FL and unveiled the expression levels of genes drd2b, 5-ht2c and abcc2 as possible markers of exposure to FL.

Mário C. C. de Pinna Robert H. Gibbs, Jr. Memorial Award for Excellence in Systematic Ichthyology- 2020

Mário C. C. de Pinna, the winner of Robert H. Gibbs, Jr. Memorial Award for Excellence in Systematic Ichthyology, is featured. De Pinna's research interests run the gamut of systematic ichthyology, from phylogenetic relationships, taxonomy, and biogeography, to theoretical considerations of phylogeny reconstruction, species concepts, the history of comparative biology and its place within the sciences. And his contributions across each of these arenas have been profound and impactful. Indeed, while still a graduate student in the joint City University of New York and American Museum of Natural History program, they published a paper on "Concepts and tests of homology in the cladistic paradigm," a contribution that has been cited over 1,093 times since its publication and almost two decades later has been cited 90 times in the past five years alone. His professional career began while an undergraduate with a publication on a cave population of Trichomyctems, and in the ensuing years has published over 90 peer-reviewed papers. Most of these have focused on the broad sweep of catfish systematics from the resolution of basal relationships across the order to numerous analyses of the composition and inter- and intrarelationships of individual catfish families and genera.

COVID-19 pandemic impacts on global inland fisheries.

The COVID-19 pandemic has led to environmental recovery in some ecosystems from a global "anthropause," yet such evidence for natural resources with extraction or production value (e.g., fisheries) is limited. This brief report provides a data-driven global snapshot of expert-perceived impacts of COVID-19 on inland fisheries. We distributed an online survey assessing perceptions of inland fishery pressures in June and July 2020 to basin-level inland fishery experts (i.e., identified by the Food and Agriculture Organization of the United Nations across the global North and South); 437 respondents from 79 countries addressed 93 unique hydrological basins, accounting for 82.1% of global inland fish catch. Based on the responses analyzed against extrinsic fish catch and human development index data, pandemic impacts on inland fisheries 1) add gradation to the largely positive environmental narrative of the global pandemic and 2) identify that basins of higher provisioning value are perceived to experience greater fishery pressures but may have limited compensatory capacity to mitigate COVID-19 impacts along with negative pressures already present.